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empty vector control  (OriGene)


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    Structured Review

    OriGene empty vector control
    Empty Vector Control, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/empty+vector/Parp11+(NM_181402)+Mouse+Tagged+ORF+Clone/bio_rxiv__64898__2026__06__01__729331-208-6-12
    Average 94 stars, based on 1 article reviews
    empty vector control - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Transfection:

    Article Title: BRSK2 plays a role in autophagy and cancer cell growth and survival under nutrient deprivation stress via the PIK3C3 pathway.
    Article Snippet: .. After 24 h of transfection, cells were again transfected with the BRSK2 plasmid (0.75 μg/well; 1 × 106 cells) and empty vector (OriGene, PS100001). ..

    Article Title: The developmental regulator HAND1 inhibits gastric carcinogenesis through enhancing ER stress apoptosis via targeting CHOP and BAK which is augmented by cisplatin.
    Article Snippet: .. HAND1-expressing plasmid and cell transfection GC cell line AGS and MKN28 were transfected with pCMV6-Entry HAND1 plasmid or empty vector (pCMV6-EntryVector) (Origene, Rockville, MD) as control, using LipofectamineTM 3000 transfection reagent (Invitrogen, Carlsbad, CA). ..

    Article Title: Expression of ZKSCAN3 protein suppresses proliferation, migration, and invasion of pancreatic cancer through autophagy
    Article Snippet: Selection of stable clones was carried out by treating each PaCa cell line with puromycin (CAS 58–58‐2; cat. no. sc‐108,071; Santa Cruz Biotechnology, Inc.) at the concentrations stated below: Specifically, PANC‐1 was treated at 5 μg/mL, MIA PaCa‐2 at 1 μg/mL, and SW 1990 at 1 μg/mL. .. Conversely, the ZKSCAN3 expression construct (RC202971; OriGene Technologies, Inc.) or empty vector (PS100001; OriGene Technologies, Inc.) was transfected into the pancreatic cancer cell line BxPC‐3 using jetOPTIMUS (Funakoshi Co., Ltd.). .. To obtain stable clones, these cells were treated with 100 μg/mL G418 (cat. no. 10131035; Gibco/Thermo Fisher Scientific, Inc.).

    Article Title: Induction of interleukin-6 by SPZ1-mediated Wnt5a signaling boosts progression of nasopharyngeal carcinoma cells
    Article Snippet: .. Cells were transfected with the plasmid carrying full-length human SPZ1 cDNA (GenBank NM 032567.2) or empty vector (OriGene Technologies Inc) using LipofectamineTM 2000 reagent (Invitrogen) according to the manufacturer's instructions. .. The stably transfected cells were screened with 800μg/ml G418 sulfate (Sigma) .

    Plasmid Preparation:

    Article Title: BRSK2 plays a role in autophagy and cancer cell growth and survival under nutrient deprivation stress via the PIK3C3 pathway.
    Article Snippet: .. After 24 h of transfection, cells were again transfected with the BRSK2 plasmid (0.75 μg/well; 1 × 106 cells) and empty vector (OriGene, PS100001). ..

    Article Title: The developmental regulator HAND1 inhibits gastric carcinogenesis through enhancing ER stress apoptosis via targeting CHOP and BAK which is augmented by cisplatin.
    Article Snippet: .. HAND1-expressing plasmid and cell transfection GC cell line AGS and MKN28 were transfected with pCMV6-Entry HAND1 plasmid or empty vector (pCMV6-EntryVector) (Origene, Rockville, MD) as control, using LipofectamineTM 3000 transfection reagent (Invitrogen, Carlsbad, CA). ..

    Article Title: Expression of ZKSCAN3 protein suppresses proliferation, migration, and invasion of pancreatic cancer through autophagy
    Article Snippet: Selection of stable clones was carried out by treating each PaCa cell line with puromycin (CAS 58–58‐2; cat. no. sc‐108,071; Santa Cruz Biotechnology, Inc.) at the concentrations stated below: Specifically, PANC‐1 was treated at 5 μg/mL, MIA PaCa‐2 at 1 μg/mL, and SW 1990 at 1 μg/mL. .. Conversely, the ZKSCAN3 expression construct (RC202971; OriGene Technologies, Inc.) or empty vector (PS100001; OriGene Technologies, Inc.) was transfected into the pancreatic cancer cell line BxPC‐3 using jetOPTIMUS (Funakoshi Co., Ltd.). .. To obtain stable clones, these cells were treated with 100 μg/mL G418 (cat. no. 10131035; Gibco/Thermo Fisher Scientific, Inc.).

    Article Title: Expression of ZKSCAN3 protein suppresses proliferation, migration, and invasion of pancreatic cancer through autophagy.
    Article Snippet: Selection of stable clones was carried out by treating each PaCa cell line with puromycin (CAS 58–58- 2; cat. no. sc- 108,071; Santa Cruz Biotechnology, Inc.) at the concentrations stated below: Specifically, PANC- 1 was treated at 5 μg/mL, MIA PaCa- 2 at 1 μg/mL, and SW 1990 at 1 μg/mL. .. Conversely, the ZKSCAN3 expression construct (RC202971; OriGene Technologies, Inc.) or empty vector (PS100001; OriGene Technologies, Inc.) was K E Y W O R D S autophagy, cancer, pancreatic cancer, zinc finger protein, ZKSCAN3 nloaded from https://onlinelibrary.w iley.com /doi/10.1111/cas.16173 by < Shibboleth> -m em ber@ bcu.ac.uk, W iley O nline L ibrary on [01/05/2024]. .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense transfected into the pancreatic cancer cell line BxPC- 3 using jetOPTIMUS (Funakoshi Co., Ltd.).

    Article Title: Modulating ACVRL1 Expression in HMEC1 Cells as a Simplified In Vitro Model for Hereditary Hemorrhagic Telangiectasia (HHT) Type 2 Studies.
    Article Snippet: A total of 2.4x105 cells were harvested for transfection and incubated in 390 μl hypoosmolar buffer (90 mOsmol/kg, #732-6007, Eppendorf) together with 8 μg of an ACVRL1-wildtype encoding plasmid (pCMV6-AC ACVRL1, #SC321860, Origene, Rockville, FL, USA). .. The respective empty vector (pCMV6-AC, #PS100020, Origene) was used as a negative control. .. A total volume of 400 μl was transferred into cuvettes with a 2 mm electrode slit (#4307000593, Eppendorf).

    Article Title: Induction of interleukin-6 by SPZ1-mediated Wnt5a signaling boosts progression of nasopharyngeal carcinoma cells
    Article Snippet: .. Cells were transfected with the plasmid carrying full-length human SPZ1 cDNA (GenBank NM 032567.2) or empty vector (OriGene Technologies Inc) using LipofectamineTM 2000 reagent (Invitrogen) according to the manufacturer's instructions. .. The stably transfected cells were screened with 800μg/ml G418 sulfate (Sigma) .

    Control:

    Article Title: The developmental regulator HAND1 inhibits gastric carcinogenesis through enhancing ER stress apoptosis via targeting CHOP and BAK which is augmented by cisplatin.
    Article Snippet: .. HAND1-expressing plasmid and cell transfection GC cell line AGS and MKN28 were transfected with pCMV6-Entry HAND1 plasmid or empty vector (pCMV6-EntryVector) (Origene, Rockville, MD) as control, using LipofectamineTM 3000 transfection reagent (Invitrogen, Carlsbad, CA). ..

    Expressing:

    Article Title: Expression of ZKSCAN3 protein suppresses proliferation, migration, and invasion of pancreatic cancer through autophagy
    Article Snippet: Selection of stable clones was carried out by treating each PaCa cell line with puromycin (CAS 58–58‐2; cat. no. sc‐108,071; Santa Cruz Biotechnology, Inc.) at the concentrations stated below: Specifically, PANC‐1 was treated at 5 μg/mL, MIA PaCa‐2 at 1 μg/mL, and SW 1990 at 1 μg/mL. .. Conversely, the ZKSCAN3 expression construct (RC202971; OriGene Technologies, Inc.) or empty vector (PS100001; OriGene Technologies, Inc.) was transfected into the pancreatic cancer cell line BxPC‐3 using jetOPTIMUS (Funakoshi Co., Ltd.). .. To obtain stable clones, these cells were treated with 100 μg/mL G418 (cat. no. 10131035; Gibco/Thermo Fisher Scientific, Inc.).

    Article Title: Expression of ZKSCAN3 protein suppresses proliferation, migration, and invasion of pancreatic cancer through autophagy.
    Article Snippet: Selection of stable clones was carried out by treating each PaCa cell line with puromycin (CAS 58–58- 2; cat. no. sc- 108,071; Santa Cruz Biotechnology, Inc.) at the concentrations stated below: Specifically, PANC- 1 was treated at 5 μg/mL, MIA PaCa- 2 at 1 μg/mL, and SW 1990 at 1 μg/mL. .. Conversely, the ZKSCAN3 expression construct (RC202971; OriGene Technologies, Inc.) or empty vector (PS100001; OriGene Technologies, Inc.) was K E Y W O R D S autophagy, cancer, pancreatic cancer, zinc finger protein, ZKSCAN3 nloaded from https://onlinelibrary.w iley.com /doi/10.1111/cas.16173 by < Shibboleth> -m em ber@ bcu.ac.uk, W iley O nline L ibrary on [01/05/2024]. .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense transfected into the pancreatic cancer cell line BxPC- 3 using jetOPTIMUS (Funakoshi Co., Ltd.).

    Construct:

    Article Title: Expression of ZKSCAN3 protein suppresses proliferation, migration, and invasion of pancreatic cancer through autophagy
    Article Snippet: Selection of stable clones was carried out by treating each PaCa cell line with puromycin (CAS 58–58‐2; cat. no. sc‐108,071; Santa Cruz Biotechnology, Inc.) at the concentrations stated below: Specifically, PANC‐1 was treated at 5 μg/mL, MIA PaCa‐2 at 1 μg/mL, and SW 1990 at 1 μg/mL. .. Conversely, the ZKSCAN3 expression construct (RC202971; OriGene Technologies, Inc.) or empty vector (PS100001; OriGene Technologies, Inc.) was transfected into the pancreatic cancer cell line BxPC‐3 using jetOPTIMUS (Funakoshi Co., Ltd.). .. To obtain stable clones, these cells were treated with 100 μg/mL G418 (cat. no. 10131035; Gibco/Thermo Fisher Scientific, Inc.).

    Article Title: Expression of ZKSCAN3 protein suppresses proliferation, migration, and invasion of pancreatic cancer through autophagy.
    Article Snippet: Selection of stable clones was carried out by treating each PaCa cell line with puromycin (CAS 58–58- 2; cat. no. sc- 108,071; Santa Cruz Biotechnology, Inc.) at the concentrations stated below: Specifically, PANC- 1 was treated at 5 μg/mL, MIA PaCa- 2 at 1 μg/mL, and SW 1990 at 1 μg/mL. .. Conversely, the ZKSCAN3 expression construct (RC202971; OriGene Technologies, Inc.) or empty vector (PS100001; OriGene Technologies, Inc.) was K E Y W O R D S autophagy, cancer, pancreatic cancer, zinc finger protein, ZKSCAN3 nloaded from https://onlinelibrary.w iley.com /doi/10.1111/cas.16173 by < Shibboleth> -m em ber@ bcu.ac.uk, W iley O nline L ibrary on [01/05/2024]. .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense transfected into the pancreatic cancer cell line BxPC- 3 using jetOPTIMUS (Funakoshi Co., Ltd.).

    Over Expression:

    Article Title: SOX5 inhibition overcomes PARP inhibitor resistance in BRCA-mutated breast and ovarian cancer.
    Article Snippet: .. Plasmids and ectopic overexpression studies The SOX5 overexpression vector, (cat# RC224228) and the respective empty vector (cat# PS100001) were purchased from Origene (Rockville, MD, USA). .. The pEGFP-C3-hYAP1 (Addgene plasmid #17843) overexpression vector was purchased from Addgene (Watertown, MA, USA).

    other:

    Article Title: The CEMIP Hyaluronidase is Elevated in Oligodendrocyte Progenitor Cells and Inhibits Oligodendrocyte Maturation.
    Article Snippet: Sections were hybridized at 40 �C with probe mixtures targeting combinations of CEMIP and control probes (ACD).

    Negative Control:

    Article Title: Modulating ACVRL1 Expression in HMEC1 Cells as a Simplified In Vitro Model for Hereditary Hemorrhagic Telangiectasia (HHT) Type 2 Studies.
    Article Snippet: A total of 2.4x105 cells were harvested for transfection and incubated in 390 μl hypoosmolar buffer (90 mOsmol/kg, #732-6007, Eppendorf) together with 8 μg of an ACVRL1-wildtype encoding plasmid (pCMV6-AC ACVRL1, #SC321860, Origene, Rockville, FL, USA). .. The respective empty vector (pCMV6-AC, #PS100020, Origene) was used as a negative control. .. A total volume of 400 μl was transferred into cuvettes with a 2 mm electrode slit (#4307000593, Eppendorf).



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    Image Search Results


    a, HCT116 cells stably expressing BRIP1 R162Q were transiently transfected using PEI with empty vector (EV-EGFP) or RNaseH1-WT-eGFP. Transfection efficiency was assessed via EGFP fluorescence. b, immunoblot analysis confirming expression of GFP-tagged constructs in transfected cells. c, Immunofluorescence detection of R-loops using the S9.6 antibody in cells transfected with EGFP or EGFP-RNaseH1. d, Quantification of S9.6 mean fluorescence intensity (MFI) per nucleus. Data represent mean ± SEM of three independent experiments. e, Immunofluorescence detection of G-quadruplex (G4) structures using the BG4 antibody in cells transfected with EGFP or EGFP-RNaseH1. f, Quantification of BG4 fluorescence intensity per nucleus. Data represent mean ± SEM of three independent experiments. g, Representative DNA fiber assay images from HCT116 cells harboring BRIP1 R162Q transfected with empty vector (EV-eGFP) or RNaseH1-WT. DNA replication tracts were sequentially labeled with CldU (red) and IdU (green). h, Quantification of DNA fiber types. RNaseH1-WT expression reduced the proportion of stalled replication forks in cells harboring BRIP1 R162Q. i, While the overall fork speed was not significantly increased upon RNaseH1-WT expression, tracts displayed a more balanced distribution of CldU and IdU incorporation. j, Fork asymmetry analysis. RNaseH1-WT expression reduced fork asymmetry, indicating that left- and right-moving sister forks progressed at more similar speeds. Labeled tracks were visualized by confocal microscopy, and tract lengths were measured on raw LSM images. Lengths (µm) were converted to replication speed (kb/min) using a factor of 2.59 kb/µm. All data represent mean ± SEM from three independent experiments. At least 150 fibers per condition were analyzed in each replicate. Statistical significance was determined using paired two-tailed Student’s t-test (*p < 0.05, **p < 0.01, ***p < 0.001, ns = not significant). c, e, Representative confocal images are shown.

    Journal: bioRxiv

    Article Title: Role of a childhood cancer-linked BRIP1/FANCJ germline variant in genomic instability and cancer cell vulnerability

    doi: 10.64898/2026.03.24.714005

    Figure Lengend Snippet: a, HCT116 cells stably expressing BRIP1 R162Q were transiently transfected using PEI with empty vector (EV-EGFP) or RNaseH1-WT-eGFP. Transfection efficiency was assessed via EGFP fluorescence. b, immunoblot analysis confirming expression of GFP-tagged constructs in transfected cells. c, Immunofluorescence detection of R-loops using the S9.6 antibody in cells transfected with EGFP or EGFP-RNaseH1. d, Quantification of S9.6 mean fluorescence intensity (MFI) per nucleus. Data represent mean ± SEM of three independent experiments. e, Immunofluorescence detection of G-quadruplex (G4) structures using the BG4 antibody in cells transfected with EGFP or EGFP-RNaseH1. f, Quantification of BG4 fluorescence intensity per nucleus. Data represent mean ± SEM of three independent experiments. g, Representative DNA fiber assay images from HCT116 cells harboring BRIP1 R162Q transfected with empty vector (EV-eGFP) or RNaseH1-WT. DNA replication tracts were sequentially labeled with CldU (red) and IdU (green). h, Quantification of DNA fiber types. RNaseH1-WT expression reduced the proportion of stalled replication forks in cells harboring BRIP1 R162Q. i, While the overall fork speed was not significantly increased upon RNaseH1-WT expression, tracts displayed a more balanced distribution of CldU and IdU incorporation. j, Fork asymmetry analysis. RNaseH1-WT expression reduced fork asymmetry, indicating that left- and right-moving sister forks progressed at more similar speeds. Labeled tracks were visualized by confocal microscopy, and tract lengths were measured on raw LSM images. Lengths (µm) were converted to replication speed (kb/min) using a factor of 2.59 kb/µm. All data represent mean ± SEM from three independent experiments. At least 150 fibers per condition were analyzed in each replicate. Statistical significance was determined using paired two-tailed Student’s t-test (*p < 0.05, **p < 0.01, ***p < 0.001, ns = not significant). c, e, Representative confocal images are shown.

    Article Snippet: For R-loop removal, cells were transiently transfected with an EGFP empty vector control (pEGFP-C1, Takara Bio, #632470), EGFP-tagged RNaseH1 wild-type (WT), or the catalytically inactive RNaseH1 D145N mutant as indicated.

    Techniques: Stable Transfection, Expressing, Transfection, Plasmid Preparation, Fluorescence, Western Blot, Construct, Immunofluorescence, Labeling, Confocal Microscopy, Two Tailed Test